From lab to field: the case study of molecular tools for marker assisted breeding in pea virus and fungal resistances
2008
Smýkal, P., Argitec Plant Research Ltd., Sumperk (Czech Republic)
The incorporation of marker assisted selection (MAS) into breeding strategies would result in reduction in the number of offspring to be propagated, selected and tested. While traditional plant breeding relies upon crosses and subsequent selection of genotypes to meet desirable trait such as yield, quality and disease resistances. MAS currently leans on statistical testing of associations between DNA markers and chosen trait and only in few cases when specific allele of gene of interest was identified, direct assisted selection of desirable combinations of genes is possible. In case of pea (Pisum sativum) the testing of resistance to viruses such as Pea Seed-borne Mosaic Virus (PSbMV) and fungus powdery mildew (Erisiphe pisi) pathogens, is included in breeding process. The resistance to PSbMV is conferred by a single recesive sbm-1 or er-1 locus in case of mildew, localized on LG VI and IV respectively. This analysis led to identification of novel alleles. Subsequently, PCR-based, both single nucleotide polymorphism (SNP) and co-dominant amplicon length polymorphism markers were developed and proved to be fully reliable on broad spectra of pea varieties and breeding lines. All these data will be discussed in light of breeding process applications, aiming at selection efficiency improvement by circumvention of the need for laborious and time consuming symptomatic testing.
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