Izolation of DNA for fragment analyses from tomato leaves and seed
2011
Miskoska-Milevska, E., Faculty of Agricultural Sciences and Food, Skopje (The Former Yugoslav Republic of Macedonia) | Popovski, Z.T. | Dimitrievska, B.R. | Porcu, K.D.
An isolation of DNA from plant tissues is problematic and often time-consuming process due to the presence of secondary metabolites as well as cell wall. In this survey the isolation of DNA for fragment analysis from tomato leaves and seeds was studied. Six different tomato varieties from three subspecies of Lycopersicon esculentum were included in this research: var. grandifolium from subsp. cultum; var. cerasiforme (with red fruits), var. cerasiforme (with yellow fruits), var. pruniforme and var. pyriforme from subsp. subspontaneum and var. racemigerum from subsp. spontaneum. Two methods for DNA isolation were examined in this study: Wizard Genomic DNA purification kit from Promega and CTAB method modified during this research. Based on the analysis of integriti of isolated DNA, it was concluded that Wizard Genomic DNA purification kit from Promega showed satisfactory results for DNA isolation from tomato leaves, and modified CTAB method for DNA isolation from tomato seeds. The isolated DNA samples were of high quality and suitable for PCR restrictions and further for DNA fragment analysis.
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