Validation of total combustion method for crude proteins determination in animal feed | Validacija metode totalnog sagorevanja za određivanje sirovih proteina u hrani za životinje
2009
Jakšić, S., Naučni institut za veterinarstvo Novi Sad, Novi Sad (Serbia) | Mihaljev, Ž., Naučni institut za veterinarstvo Novi Sad, Novi Sad (Serbia) | Živkov-Baloš, M., Naučni institut za veterinarstvo Novi Sad, Novi Sad (Serbia) | Mašić, Z., Poljoprivredni fakultet, Novi Sad (Serbia)
Crude proteins content is one of the most important parameters of animal feed quality. For total proteins determination Kjeldahl method is the most known and most used. However, total combustion method, well-known as Dumas, is also recognized as standard method. In this paper the model of Dumas validation for total combustion method on the instrument Elementar- Rapid N is shown. The validation involves determination of linearity, detection limit, accuracy and precision, as the standard required. In validation procedure standard substances were used and the results were processed with suitable statistics methods in Microsoft Excel. Examined validation parameters show that the tested method can be used for accurate determination of total nitrogen (protein) determination in animal feed samples.
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