Microsatellite analysis of Valachian sheep population in Slovakia using 16-plex PCR | Analýza mikrosatelitov v populácii oviec plemena valaška na Slovensku pomocou 16-plex PCR
2010
Bauer, M., Animal Production Research Centre, Nitra (Slovak Republic), Department of Genetics and Animal Reproduction | Margetín, M., Slovak Agricultural Univ. in Nitra (Slovak Republic) | Bauerová, M., Univerzita Konštantína Filozofa, Nitra (Slovak Republic) | Oravcová, M., Animal Production Research Centre, Nitra (Slovak Republic) | Peškovičová, D., Animal Production Research Centre, Nitra (Slovak Republic)
The genetic variability of Valachian sheep population in Slovakia was tested using 16 microsateilite markers in a single multiplex PCR. Fourty-seven animals from three different regions of Slovakia were genotyped. All loci were highly polymorphic, showing between 7 (MAF65, SPS113) and 15 atleles (TCRGC4), PICs between 0.666 (SPS113), and 0.894 (TCRGC4). Estimates of expected heterozygosity (He) ranged between 0.581 for SPS113 to 0.871 for TCRGC4. The average He over all loci was 0.779, and the average observed heterozygosity (Ho) over all loci was lower, i.e. 0.719. There was not observed any difference in genetic variability among sub-populations from different regions. Estimates of He over all loci were 0.753, 0.744, and 0.798; whereas Ho values were 0.700, 0.677, and 0.778 in sub-population from Spiš, Kysuce, and Liptov.
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