Influence of different factors on tannins and flavonoids extraction of some thyme varieties representatives of thymol, geraniol and citral chemotype
2012
Zhekova, G., Institute for Roses, Kazanlak (Bulgaria) | Pavlov, D., Thracian University, Stara Zagora (Bulgaria)
Three chemotype thyme – thymol Nemska zima, citral – Slava and geraniol – Pagane with purpose obtaining of extract for cosmetics and pharmaceutics application were investigated in the present work. The extraction was carried out as stationary periodical process with ratio between dry herb and solvent 1:10, temperature from 20ºC to 60ºC, duration from 1 to 5 hours with solvents – 60% ethanol and 85% glycerin. The obtained extracts were determined the content of tannins and flavonoids. The data were presented as four factors analysis – type of solvent, thyme variety, temperature and duration of process. The results showed that the thyme variety has the strongest influence on extracted tannins and flavonoids, followed by solvent type, temperature and duration of the process. The extract obtained from variety Nemska zima has the highest content of derived substances followed by Slava and Pagane. The ethanol derives tannins and flavonoids in bigger extent than the glycerin. The differences between the two solvents are most significant at temperature 20ºC.
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