Improvement of a system of selection of initial material for strain renovation of the base collection of potato varieties in vitro | Усовершенствование системы отбора исходного материала для сортообновления базисной коллекции сортов картофеля в условиях in vitro
2011
Adamova, A.I. | Radkovich, E.V. | Gushcha, G.N., National Academy of Sciences of Belarus, Minsk (Belarus). Scientific and Practical Center for Potato, Vegetable and Fruit Growing
Selection of high-productive clones of recognized potato (Solanum tuberosum) varieties which follow through with morphological traits and clear of silent infection of virus and bacilliform diseases for micro-cloning and depositing was realized in the central part of the Republic of Belarus in 2006-2010. Qualitative complex system of selection of parental plants, directed not on the recovery, but on search of healthy plants, confirmed by testing for the presence of pathogens on the state of the index, followed by their transfer into culture in vitro, allows to exclude a number of procedures connected with rehabilitation, as well as the influence of inhibitory preparations on the genotype of the plant. In course of the study there was taken into account the conducted research and literary facts of efficiency of cultivation terms of one and the same lines on growing medium without harm to varietal traits and quality, as well as the fact that the annual testing of the basic collection of potato before the beginning of a mass microclonal propagation lowered collection potato amount. That is why it was decided to realize strain renovation every 4 years. Scheme of clone selection of recognized potato varieties for strain renovation of the base collection on the field nurseries using enzyme immunoassay and PCR method in complex indexation made it possible to select healthy initial material for the further in vitro and micro-cloning propagation. Disease testing at the plant-indices level (inclusive the clone selection along with complex diagnostics and further introduction of healthy clones into tissue culture) takes 9-10 months. For the range extension of healthy initial material there was increased number of clones from 20 to 100 in each variety.
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