Study on the substrate inhibition and the method of disarming inhibition in the fermentation process of hyaluronic acid | 川稿佃绋涓╂跺瑙ｉゆ剁规绌
2011
She Yonghong, Ningxia University, Yinchuan (China), College of Agriculture | Luo Ruiming, Ningxia University, Yinchuan (China), College of Agriculture | Liu Xiaolian, Ningxia University, Yinchuan (China), College of Agriculture
[Objective] The research aimed to search and obtain the fermentation method of hyaluronic acid with the high yield. [Method] In the production process of hyaluronic acid by the microbial fermentation method, the influence of substrate concentration on the fermentation was explored. [Result] The glucose had the biggest influence on the hyaluronic acid fermentation. The low concentration of glucose only could synthetize a little bacteria and hyaluronic acid, and the high concentration of glucose could inhibit the growth of bacteria and the formation of hyaluronic acid. Via the exploratory experiment, the fed-batch fermentation could disarm the substrate inhibition. The concrete method was adding 2% glucose before the start of fermentation, and adding 2% glucose at the 14th hour of fermentation, and adding 2% glucose again at the 22nd hour of fermentation. [Conclusion] In the microbial fermentation process, the fed-batch fermentation could disarm the substrate inhibition and obtain the hyaluronic acid with the larger relative molecular mass and the higher yield.
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