Effect of salt tolerance of DNA of Dunaliella salina on soybean seedling_ | 盐藻DNA对大豆幼苗耐盐性的影响___
2011
Guo Jinyao, Huaihai Institute of Technology, Lianyungang (China) ，School of Marine Science and Technology | Yang Xiaoling, Huaihai Institute of Technology, Lianyungang (China) ，School of Marine Science and Technology
chinois. ［目的］增强大豆幼苗的耐盐性。［方法］用0.1 mol/L氯化钙溶液浸泡大豆种子4 h后，将其浸入5 mg/L盐藻DNA水溶液中，蒸馏水浸泡作为对照组。选取长势一致的大豆幼苗，用蒸馏水冲洗干净幼苗的根部，栽于含3 g/L氯化钠的MS培养液中，处理与对照各60株。检测大豆幼苗耐盐适应性的变化。［结果］处理组的植株鲜重和株高分别较对照高出33.6和22.1个百分点，存活率高出46.7个百分点，根数减少了26.2个百分点，根长缩短了17.6个百分点。［结论］盐藻DNA提高了大豆幼苗的耐盐性。
Afficher plus [+] Moins [-]anglais. ［Objective］ The study aimed to enhance salt tolerance of soybean. ［Method］ The soybean seeds was soaked in 0.1 mol/L calcium chloride solution for 4 hours, than immerse them in 5 mg/L DNA of Dunaliella salina solution, and soaked in distilled water as CK. Selected grew consistent soybean seedlings, rinsed clean with distilled water seedling roots and planted in 3 g/L sodium chloride MS cultures of processing and contrast, each 60 strains. Soybean seedlings salt resistance changes was detected. ［Result］The result showed that, soybean seedlings were dealt with concentration at 5 mg/L DNA of Dunaliella salina, afert cultivating for 15 days in MS culture solution with concentration at 3 mg/L NaCl, the seedling weight and seedling height have increased by 33.6% and 22.1%, the seedling survival rate have increased by 133.4%, decreased 26.2% of the number of root and shortened 17.6% the length of root. ［Conclusion］ The DNA of Dunaliella salina increased salt tolerance in soybean seedlings._
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