Mechanism of starch-sucrose metabolism regulated by iba as well as ga3 during scale cutting propagation in lilium | IBA与GA3调控百合鳞片扦插繁殖的“淀粉-蔗糖”代谢机制

chinois. 【目的】研究IBA与GA3 促进百合鳞片扦插繁殖的碳水化合物代谢机制,为调控鳞片繁殖技术、深入研究小鳞茎形成发育的生理机制奠定基础。【方法】以亚洲系‘精粹’百合为试材,设计200 mg?L-1 IBA浸泡鳞片2 h和200 mg?L-1 GA3浸泡鳞片8 h两个处理,以同体积清水浸泡鳞片2 h为对照,测定母鳞片不同部位和小鳞茎中淀粉、蔗糖含量及相关酶活性。【结果】施用IBA与GA3促进了母鳞片的淀粉降解、蔗糖合成以及鳞片上、中部的糖类物质向基部的运转,加快了小鳞茎的形态建成。母鳞片的淀粉降解部分受淀粉磷酸化酶(starch phosphorylase, SP)调控,蔗糖含量与蔗糖合成酶(sucrose synthase, SS)、蔗糖磷酸合成酶(sucrose phosphate synthase, SPS)活性极显著正相关。IBA促进鳞片淀粉降解、可溶性糖利用或运转的效用高于GA3,且IBA有利于母鳞片蔗糖的尽早合成;GA3在培养前期抑制了鳞片上部和中部的淀粉降解,并促进母鳞片合成较多的蔗糖。【结论】IBA和GA3促使母鳞片内淀粉以及蔗糖的代谢,相关酶活性提高,从而提高繁殖系数或获得最佳小鳞茎个体。

anglais. 【Objective】 The effects of IBA and GA3 on starch-sucrose metabolism during scale cutting propagation were studied with the aim to provide a theoretical and practical basis for regulating the development of bulblets and explore the physiological mechanism of scale propagation.【Method】The scales of Lilium Asiatic hybrids ‘Elite’ were immersed in IBA solution at concentration 200 mg?L-1 for 2 h and GA3 solution at concentration 200 mg?L-1 for 8 h, respectively. And the control was designed with water in same volume 2 h. The starch content, sucrose content and the activities of related enzymes in various positions of mother scales and bulblets were determined. 【Result】 Results showed that IBA and GA3 accelerated the starch degradation, sucrose synthesis and the transfer of carbohydrate from apical, central regions to basal region in mother scales. Furthermore, these substantial changes speeded up the bulblet morphopoiesis. Starch degradation was partly regulated by SP in parent scales, and the sucrose content was significantly positively correlated with the SS as well as SPS activity. The efficiency of IBA on carbohydrates metabolism was higher than that of GA3. Besides, IBA was conducive to synthesize sucrose earlier in the mother scales. By comparison, GA3 restrained the degradation of starch in the mother scales during the initial culture period and promoted more sucrose synthesis. 【Conclusion】 IBA and GA3 promoted the metabolism of starch as well as sucrose, and increased the activities of related enzymes in the parent scales, and then improved the propagation coefficient or produced the optimum bulblets.