Engineering tocopherol biosynthetic pathway in lettuce
2011
Li, Y., Fudan Univ., Shanghai (China). State Key Lab. of Genetic Engineering | Wang, G., Shanghai Jiao Tong Univ. (China). Plant Biotechnology Research Center | Hou, R., Fudan Univ., Shanghai (China). State Key Lab. of Genetic Engineering | Zhou, Y., Fudan Univ., Shanghai (China). State Key Lab. of Genetic Engineering | Gong, R., Shanghai Jiao Tong Univ. (China). Plant Biotechnology Research Center | Sun, X., Fudan Univ., Shanghai (China). State Key Lab. of Genetic Engineering | Tang, K., Fudan Univ., Shanghai (China). State Key Lab. of Genetic Engineering
In order to increase tocopherol content, genes encoding Arabidopsis homogentisate phytyltransferase (HPT) and gamma-tocopherol methyltransferase (gamma-TMT) were constitutively over-expressed in lettuce (Lactuca sativa var. logifolia), alone or in combination. Over-expression of hpt could increase total tocopherol content, while over-expression of gamma-TMT could shift tocopherol composition in favor of alpha-tocopherol. Transgenic lettuce lines expressing both hpt and gamma-TMT produced significantly higher amount of tocopherol and elevated alpha-/gamma-tocopherol ratio compared with non-transgenic control and transgenic lines harboring a single gene (hpt or gamma-TMT). The best line produced eight times more tocopherol than the non-transgenic control and more than twice than hpt single-gene transgenic line.
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