High frequency plant regeneration from the mature seeds of Garcinia indica
2011
Baskaran, M., Goa Univ. (India). Dept. of Botany | Krishnan, S., Goa Univ. (India). Dept. of Botany
A high frequency plant regeneration system was developed for the production of high yielding elite clones of Garcinia indica via direct organogenesis. A maximum number of 86.2 shoot buds per explant were induced from the mature seed segments cultured on Woody plant medium (WPM) supplemented with 2.0 mg/cubic dm N6-benzyladenine and 1 mg/cubic dm indole-3-acetic acid. Rooting was achieved on half-strength WPM medium supplemented with 3 mg/cubic dm indole-3-butyric acid. Rooted plantlets were acclimatized and transferred to greenhouse for further growth. The highest survival rate of 95 % was recorded using a mixture of garden soil and sand. Histological studies clearly revealed multiple shoot formation from mature seed segments.
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