Application of simplified-AFLP to assess genetic diversity of Pseudomonas syringae pv. syringae strains isolated from rice and wheat hosts
2011
Habibi, R., University of Guilan, Rasht (Iran Islamic Republic). Dept. of Plant Protection | Niknejhad Kazempoor, M., University of Guilan, Rasht (Iran Islamic Republic). Dept. of Plant Protection | Elahinia, A., University of Guilan, Rasht (Iran Islamic Republic). Dept. of Plant Protection | Rabiei, B., University of Guilan, Rasht (Iran Islamic Republic). Faculty of Agriculture | Ebadi, A., Rice Research Inst. of Iran, Rasht (Iran Islamic Republic)
Pseudomonas syringae pv. syringae is a plant pathogen bacterium causing bacterial leaf blight in wheat and sheath rot in rice. In this study, simplified AFLP was tested in order to evaluate genetic diversity of 60 P.s. pv. syringae strains isolated from wheat and rice fields of Guilan province-Iran by 12 AFLP primers. The products resulting from AFLP were separated using agarose gel electrophoresis. The maximum number of PIC among all tested primers belonged to primer 36. Based on UPGMA method, using NTSISpc. software, all P.s. pv. syringae strains were divided to four distinct clusters at 70% similarity level. Genetic analysis of studied populations (pop1: isolates of rice field, pop2: isolates of wheat, pop3: isolates of rice panicle, pop4: isolates of rice nursery) determined that the high genetic similarity was seen among isolates obtained from wheat and rice panicle, while strains isolated from field of rice had the most genetic distance with the other populations. Results of this study showed that AFLP is an effective marker in evaluating genetic diversity within and among studied populations while all of them had the same pathogenesis characteristics.
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