The MRN complex of wheat
2011
De Bustos, A., University of Alcala, Alcala de Henares (Spain). Dept. of Cell Biology and Genetics | Perez, R., University of Alcala, Alcala de Henares (Spain). Dept. of Cell Biology and Genetics | Cuadrado, A., University of Alcala, Alcala de Henares (Spain). Dept. of Cell Biology and Genetics | Jouve, N., University of Alcala, Alcala de Henares (Spain). Dept. of Cell Biology and Genetics
The MRN complex is formed by the interaction of the products of the Mre11, Rad50 and Nbs1 genes. This complex plays a central role on repair of double-strand breaks (DSBs) and acts in a great number of cellular processes. In this study we have performed the analysis of the MRN complex in diploid and polyploid species of wheat. The molecular characterization was carried out in the diploid T. monococcum (genome A) and Ae. tauschii (genome D) and in the tetraploid T. turgidum (genomes A and B). The results obtained showed that in all cases the genes presented the main characteristics previously described in other species. A modified FISH protocol was used to locate the Rad50, Mre11 and the Nbs1 genes on the homoeologous chromosomes 5, 2 and 1, respectively. Analysis of expression showed that the hexaploid T. aestivum was the species with the higher level of expression whereas the rest of the species analysed showed no relation with its ploidy. Also, quantification of the expression of each homoeologous gene in the polyploid species evidenced in some cases a process of silencing after polyploidization. The study of the interaction between the proteins demonstrated that the interaction of proteins was not restricted to each genome, detecting interaction between proteins belonging to different genomes.
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