Microsatellite variability in nutria (Myocastor coypus) genetic resource in the Czech Republic
2012
Kaplanova, K., Mendelova Univ., Brno (Czech Republic). Ustav Morfologie, Fyziologie a Genetiky Zvirat | Putnova, L., Mendelova Univ., Brno (Czech Republic). Ustav Morfologie, Fyziologie a Genetiky Zvirat | Bryndova, M., Mendelova Univ., Brno (Czech Republic). Ustav Morfologie, Fyziologie a Genetiky Zvirat | Bartonova, P., Mendelova Univ., Brno (Czech Republic). Ustav Morfologie, Fyziologie a Genetiky Zvirat | Vrtkova, I., Mendelova Univ., Brno (Czech Republic). Ustav Morfologie, Fyziologie a Genetiky Zvirat | Dvorak, J., Mendelova Univ., Brno (Czech Republic). Ustav Morfologie, Fyziologie a Genetiky Zvirat
The objective of our investigation was to establish microsatellite panel for nutria and determine microsatellite variability in the Czech nutria population, levels of genetic diversity within and among subpopulations with respect to colour form and the levels of inbreeding within subpopulations. Three colour forms (Standard, Moravian Silver, and Prestice Multicolour) fall into the Farm Animal Genetic Resources of the Czech Republic. The genetic variability was studied in 64 animals by investigating 11 microsatellite markers (McoD214, McoD217, McoD59, McoD69, McoC124, McoC203, McoD60, McoB17, McoC118, McoA04, and McoD228) analysed by multicoloured capillary electrophoresis. The whole population showed a moderate level of genetic variability in terms of number of alleles (5 alleles per locus) or heterozygosity (50.4%). The Prestice Multicolour form exhibited the highest level of diversity. The Standard colour form showed the highest level of inbreeding. High combined exclusion probability values (99.5, 94.5, and 99.9%) implied that the panel of microsatellite markers established in this study was usable for individual identification or routine parentage testing in nutria population in the Czech Republic.
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