New polymorphic microsatellite loci for Theobroma cacao: isolation and characterization of microsatellites from enriched genomic libraries
2012
Santos, E.S.L., Universidade Estadual do Sudoeste da Bahia, Itapetinga (Brazil). Departamento de Estudos Basicos e Instrumentais | Cerqueira-Silva, C.B.M., Universidade Estadual do Sudoeste da Bahia, Itapetinga (Brazil). Departamento de Estudos Basicos e Instrumentais | Mori, G.M., Universidade Estadual de Campinas (Brazil). Centro de Biologia Molecular e Engenharia Genetica | Ahnert, D., Universidade Estadual de Santa Cruz (Brazil). Centro de Biotecnologia e Genetica | Correa, R.X., Universidade Estadual de Santa Cruz (Brazil). Centro de Biotecnologia e Genetica | Souza, A.P., Universidade Estadual de Campinas (Brazil). Centro de Biologia Molecular e Engenharia Genetica
Seventeen polymorphic microsatellite markers were isolated from enriched genomic libraries for Theobroma cacao, providing additional tools for studying the genetic diversity and map saturation of this species. These markers were characterized in 32 accessions of the T. cacao germplasm collection from the Centro de Pesquisas do Cacau. The number of alleles at each locus varied from 2 to 8, with an average of 4.41 alleles per locus. The polymorphism information content varied from 0.060 to 0.695, with an average of 0.333.
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