In vitro induction of mutations in Dieffenbachia picta CV. tropica
2011
Abass, M.M. | El-Shamy, H.A. | Dawh, A.K.
In vitro proliferated shoots of Dieffenbachia picta cv. Tropica were subjected to chemical mutagenic treatment with N-nitroso-Nmethylurea (NMU) through either immersion of explants in different concentrations (0.0, 0.1, 0.2, 0.3 and 0.4 %) or by supplementation MS medium with different concentrations (0.0, 0.01, 0.02, 0.03, 0.04 and 0.05 %). The obtained results showed that survival percentage, number of shoots/explant and number of leaves/shoot were decreased with increasing the concentration of NMU agitation concentration. In this experiment, the lethal dose causing 50% reduction in explant survival (LD50) ranged between 0.3 - 0.4% concentrations of NMU. Increasing of NMU concentration in MS medium decreased number of shoots/explant and number of leaves/shoot without affecting the survival percentage. Screening of the second mutated generation showed that the highest NMU agitation concentration (0.4%) was efficient in increasing the number of shoots/explant. Supplementation of medium with NMU did not significantly affect the second mutated generation shoot characters except the shoot length which increased significantly by addition- of 0.03% NMU to the culture medium. Successfully acclimatized plantlets will be subjected to further screening for selection any mutated plants.
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