Genetic evaluation and rapd molecular for salt tolerance of new promising faba beanlines,Vicia faba L.
2011
Wafa, H.A.M. | Soliman, S.S.A. | Ismail, T.A. | Mahmoud, A.A.
The obtained results confirmed more variabilities between new genotypes and varities under study. The LP7 new promising line was the most salt tolerant than other genotypes, while, Giza 714 considered the most salt sensitive in all studied criteria. Osmotic pressure at 30 , 60 days and proline content recorded the highest values in LP7 and subsequently decreased in the other tested genotypes. Osmotic pressure and proline content increased with increasing of salt concentrations, especially in the most tolerant line (LP7). However, the susceptible genotypes exhibited low value of osmotic pressure and proline content. The results showed also that a positive correlation had been found between seed germination % and plant height at 30 days, osmotic pressure at 30 days and proline content under 5000 ppm. Similarly, a positive and significant correlation was found between osmotic pressure at 30 days under 5000 ppm saline and both of osmotic pressure at 60 days and proline content under 5000 ppm. Accordingly, LP7 as a tolerant line and G714 as a sensitive cultivar were crossed for study of molecular markers related to salt tolerance by using bulked segregant analysis (BSA). Bulk of the two extremely F. plant (most tolerant and most sensitive F2 groups) and two parents were used to develop some molecular genetic markers associated with salt tolerance in faba bean by using eleven RAPD primers. These results showed the possibility of genotypes for salt tolerance in breading faba bean using molecular markers.
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