Detection of Brucella melitensis from filtered bovine peripheral-blood by polymerase chain reaction test
2010
Al-Ashkar, B. | Al-Mariri, A. | Hamad, I.
Brucella melitensis contaminants of bovine peripheral-blood can be concentrated and isolated by filtration; and their DNA can be released for subsequent PCR detection by heating the isolated colony at 95°C for 10 min. Detecting rate of brucellosis in the infected sample was less than 20 CFU/ml of blood, after leukocytes filtration and culturing, as well as on isolated colonies. This PCR method also enables us to make the diagnosing of brucellosis for the animals with a negative complement
Afficher plus [+] Moins [-]Mots clés AGROVOC
Informations bibliographiques
Cette notice bibliographique a été fournie par Ain Shams University
Découvrez la collection de ce fournisseur de données dans AGRIS