Agrobacterium tumefaciens mediated transformation of cauliflower (Brassica oleracea var. botrytis) with bacterial mannitol phosphodehydrogenase gene for abiotic stress tolerance
2010
Shalini | Nambiar, A. | Chawla, H. S.
The bacterial mannitol-1- phosphodehydrogenase (mtlD) gene conferring abiotic stress resistance was introduced into cauliflower (Brassica oleracea var. botrytis) by Agrobacterium mediated transformation. Petiole and hypocotyl explants were transformed with Agrobacterium strain LBA 4404 (pCaMVMTLDS). The explants were selected on kanamycin containing medium in three selection cycles of two week duration each with low intensity of selection in first selection cycle followed by high intensity selection. After completion of three selection cycles, 9.6 and 10.6 per cent kanamycin resistant petiole and hypocotyl explants were recovered respectively. A total of 80 putative transgenic plants were regenerated from the selected explants. Thirty plants were PCR positive for both selectable marker nptII gene and mtlD gene with transformation frequency of 1.3 and 2.1 per cent from petiole and hypocotyls explants respectively.
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