Serodiagnosis of hydatidosis in buffaloes by counter immuno electrophoresis (CIE) and enzyme linked immunosorbent assay (ELISA)
2010
Sangaran, A. | John, L.
Using counter immuno electrophoresis (CIE) and enzyme linked immunosorbent assay (ELISA), 100 sera samples collected from 75 buffaloes with known hydatid cysts and 25 buffaloes without any visible hydatid cysts on slaughter were screened. By CIE, 52 sera samples were positive out of 75 buffaloes with hydatid cysts whereas 4 sera samples were detected positive from the 25 buffaloes without visible hydatid cysts. In ELISA, 65 sera samples of the 75 buffaloes with hydatid cysts proved positive and only 1 serum sample was positive from the remaining 25 sera samples screened. The sensitivity and specificity of CIE was 69% and 84% whereas in ELISA, it was 87% sensitive and 96% specific. Hence, the use of these serological tests may be of use in antemortem diagnosis of hydatidosis instead of knowing the disease only after the death of animals and confirmation on postmortem. Between the 2 serological tests used in the present study, ELISA is preferred to CIE owing to its better sensitivity and specificity and the only disadvantage with ELISA is the cost involvement. It is preferred in screening large number of sera samples rather than a few sera samples.
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