Investigation of gram negative bacteria on cotton seed.
2009
Razi Nattaj, Mohammad | Dana Far, Bahram | Azad, Qasem | Mohamud Janlu, Hojjat Ol-Lah | Rahimiyan, Heshmat Ol-Lah
Phylosphere is a site for living microorganisms including bacteria. Plants species and plant leaf type have important role on diversity and density of bacteria live on them. There are several bacteria as epiphytic on aerial parts of plants and plant seeds which cause diseases in suitable conditions. Cotton bacterial blight has been appeared as epidemic in Iran particularly in golestan province in recent years. Some suggestion propounds the presence of this bacterium on cotton seed surface. This study was conducted in Golestan province to identify the gram negative bacteria on seed surface. For this purpose samplings were done randomly from recommend seeds for planting. The seeds was incorporated into sterile distilled water and small amount of tween 20 as a detergent was added to it , and put to room temperature for one houre for better washing of bacteria on seed surface. Then the above suspention was streaked on bactewrial culture media. The growthed bacteria were isolated, purified and identified. The results revealed that Pseudomonas fluorescenc, P. syringae, Pantoea annanas and Pectobacterium sp. Were identified based on authentic references. The presence of Xanthomonas axonopodis pv. malvacearum was not proved as casual agent of bacterial blight as epiphyte on cotton seeds.
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