Transformation of wheat using fld gene for to enhance tolerance to salinity stress
2008
Tohidrar, Masoud
Abiotic stresses such as drought and salinity are the most important factors which cause the loss and reduction of yields all over the world. Respecting to complication of resistance and tolerance trait to abiotic stresses and the important role of wheat in supplying mankind food and time-consume of conventional plant breeding methods, genetic engineering is the alternative way to increase plant tolerance to abiotic stresses. In this study biolistic and Agrobacterium methods using p6U-Ubi-FVT1 vector was applied for to produce transgenic tolerant wheat against abiotic stresses .This vector containing streptomysin as a bacterial selectable marker and hygromycin as plant selectable marker.fld gene plays an isofunctional role with feredoxine which is an antioxidant and can prohibit any interruption in photosynthetic electron transfer chain and suppresses creation of reactive oxygen species. Hereby embryogenic calli of spring varieties including Bahar, Line A, Pishtaz, N-83 and winter varieties including S-87 and Zarin, were used as target explants. Result showed the most regenerartion occured in biolistic and Agrobacterium methods were Pishtaz with C58 and Bahar, respectivity. The PCR analysis and dot blat confirmed the existence of at least one copy of target gene in the wheat genome.
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