PCRIdentification of SCID and OLWS disease carriers in Arabian and Thoroughbred horses using PCR Technique
2011
Trang, A
This study aimed to identify carriers of the SCID disease in race horses Arab and the OLWS disease in race horses Thoroughbred in Iran. The inheritance pattern in both diseases is autosomal recessive. In SCID foals the number of mature B and T lymphocytes is extremely low. The foals are usually died in the months before the age of five by opportunistic infections. This anomaly occurs in the removal of 5 bp TCTCA in the gene encoding the enzyme DNA-PKcs at codon 3155, and create the change framework and cause Premature end of codon and the absence of this enzyme. Gene encoding this enzyme is located on chromosome 12p9. In this study, 228 Iranian Arab horses and 16 horses of foreign Arabs were tested using molecular techniques ARMS PCR. Four Arab horses (3 Iranians and one foreign), were diagnosed carriers and other horses were diagnosed healthy These experiment showed that frequency of this allele in the population studied is very low (1.6%) . OLWS disease is one of the genetic disease in the American paint horses. foals are completely white or almost white and die from consequences of defects of neural nodes in the intestines. The disease is caused by a two-nucleotide substitution mutations In codon 118 of the EDNRB gene on chromosome 17 horses, Which replaced the amino acid lysine (Lys) to amino acid L-Ile (Ile) . In this study, 60 Thoroughbred horses were selected. The specific primers were used to identify individual carriers (A specific primer pairs amplified L-Ile and a specific primer pairs amplified lysine).In this study all Thoroughbred horses were homozygous for the Ile118 allele This result would indicate the absence of carriers in Thoroughbred horses in the population studied.
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