Identify genes responding to salinity in sueada aegyptiaca

Salmit is one of the major constraints to crop productivity worldwide. HIgh salinity causes water deficit, ion toxidt, and nutrient deficiency leading to molecular damage. growth arrest, and even plant death. Functional analysis of the special physiology and biochemistry of halophytes that imparts their exceptional degree of salt tolerance will require the utilization of a halophytic model system. Suaeda aegp1iaca is a succulent, annual halophyic, which is native to saline soils of arid and semiarid regions of Iran. Sonic advantageous aspects of' this plant arc their high growth rate, high biomass production, and copious seed production in natural condition. On the other hand, there is a special attention to the methods that enable researchers to quantitatively study cpression levels of thousands of genes in parallel, over a time course or across a series cf defined conditions. The data obtained using these methods provides valuable information on gene function and interaction in cellular processes. In the present study, we have employed protcomics to identify the mechanisms of salt responstvcness in leaves of S aeptütca grown under different salt concentralions, Iii order to analysis the information from molecular data, whole plant growth analysis under different salt levels was also studied Tcndavold plants were treated with 0, 150, 300, 450, and 600 mM NaCI, In one exprirnent, after 40, 80 120. 160 and 200 days of treatment, plant samples were taken and plant physiological parameter were calculated. In the other, after 30 days of treatment, leaf samples were collected and analyzed using 2D-PAGE. Salt treatments changed some morphological attributes of plants and caused htf number and size to increase. Shoot dry matter increased at 150 mM NaCl, and linearly decreased froni 300 to 600 mM NaCI, Plant growth did not significantly change at l50300 mM NaCl. Physiological growth parameters, RGR and NAR, followed the same trends for different salt treatments so that the highest amount of the indices was for 150 and 300 mM NaCI, While, LWR reached the highest point in plants affected by 600 and 450 mM NaCl, respectively, Leaf Na and K contents were significantly increased and decreased, respectively, under Salinity conditions. Salinity of 150 mM had the greatest effect on increasing Na and decreasing K content in leaf tissues. Proteome analysis of leaf samples showed that out of 700 protein spots reproducible deIecTrl 'vtiications, 102 spots showed significant response to salt treatment rii,aied t 0 mM \\aC1, W analyzed e\\pression pattern of salt rcsponsi'c proteins a hlerarcluc,i and two nonhierwvhica (Furzy ART and SOM) statistical