Preparation of Conjugated Linolenic Acid from Urea Fractionated Perilla Seed Oil Hydrolysate
2011
Lee, K.S., Chungnam National University, Daejeon, Republic of Korea | Shin, J.A., Chungnam National University, Daejeon, Republic of Korea | Lee, K.T., Chungnam National University, Daejeon, Republic of Korea
Conjugated linolenic acid (CLnA) and conjugated linoleic acid (CLA) are positional geometric isomers with three and two double bonds, respectively. In this study, perilla seed oil containing 60% α-linolenic acid (C18:3) and 30% linoleic acid (C18:2) was used as a reaction substrate. After the perilla seed oil was hydrolyzed, conjugated fatty acids were synthesized using different reaction parameters, such as reaction time and concentration of sodium hydroxide. As a result, CLnA, CLA, and other newly synthesized conjugated isomers were present at levels of 14.5%, 14%, and 42.2%, respectively, when the reaction was performed with 20% NaOH, at 180℃, and for 1 hr. The results of GC-MS and fourier transform infrared spectroscopy (FT-IR) showed that CLnA isomer of cis-9, trans-11, and trans-13 octadecatrienoate, CLA isomer of cis-9, trans-11, and trans-10, cis-12 octadecadienoate, and other conjugated isomers were produced. Using urea, α-linolenic acid could be concentrated from perilla seed oil hydrolysate. After concentration by urea, the concentration of α-linolenic acid reached about 70%. After alkaline-isomerization was performed on the urea fraction containing 70% α-linolenic acid, the content of CLnA increased up to 16.6%.
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