Sequence analysis of segment A gene of a very virulent infectious bursal disease virus recently isolated in Korea
2011
Oh, H.S., Kangwon National University, Chuncheon, Republic of Korea | Lee, J.H., Kangwon National University, Chuncheon, Republic of Korea | Kwon, H.M., Kangwon National University, Chuncheon, Republic of Korea | Sung, H.W., Kangwon National University, Chuncheon, Republic of Korea
Infectious bursal disease virus (IBDV) is a member of the Avibirnavirus genus of the Birnaviridae family which genome consists of two segments (A and B) of double stranded RNA. Segment A gene of KNU08010 isolate, which was isolated from a 15-day-old chicken flock in 2008, was sequenced and compared with other IBDV isolates including SH/92 strain, the first Korean very virulent (vv) IBDV isolate. The amino acid sequences of segment A gene showed that KNU08010 had 99.2% homology with SH92 strain. KNU08010 isolate had specific amino acids A222, I242, I256, I294 and S299 which are highly conserved among vvIBDV strains. Phylogenetic analysis based on the nucleotide sequences of variable region of the VP2 gene of 18 IBDV strains revealed that KNU08010 was grouped with vvIBDVs and was closely related to Korean vvIBDVs isolated from wild birds.
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