Isolation and characterization of fowl adenovirus serotype 4 from chickens with hydropericardium syndrome in Korea
2011
Park, H.S., Kyungpook National University, Daegu, Republic of Korea | Lim, I.S., Kyungpook National University, Daegu, Republic of Korea | Kim, S.K., Kyungpook National University, Daegu, Republic of Korea | Kim, T.K., Gyeongnam Livestock Promotion Institute, Jinju, Republic of Korea | Yeo, S.G., Kyungpook National University, Daegu, Republic of Korea
Four strains of fowl adenovirus (FAdV) were isolated from 4 flocks of broiler or layer chickens affected by hydropericardium syndrome in Korea. These FAdVs were classified as serotype 4 by restriction fragment length polymorphism patterns of hexon genes and whole genomes. The virus exhibited cytopathic effects consisting of rounding, ballooning and clustering in primary chicken embryo liver cell cultures. In transmission electron microscopy, virus particles in hexagonal shape aggregated exclusively in the nuclei of hepatocytes of the chickens as the typical appearances in adenovirus infections. Buoyant density of the virus in cesium chloride (CsCl) was 1.34 g/mL. The virus was stable to chloroform, ether, 50~70% ethanol, acidic condition at pH 3, 0.25% trypsin (1 : 250), heat at 50℃ for 30 min, but labile to 100% ethanol, heat at 52~60℃ for 30 min, 1 M MgCl₂ at 50℃ for 1 h, 1 : 2,000 formalin (37%). All of the physicochemical properties pertained to the characteristics of adenoviruses. Eight viral polypeptides were determined in CsCl-purified virus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
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