Improvement of growth and nitrogen fixation of agroforest legumes through rhizobial inoculation: infection studies on Enterolobium saman (or Samanea saman) using the scanning electron microscope (SEM).
1987
Manguiat I.J.
SEM (Scanning Electron Microscope) observation on the development of root nodules of Enterolobium saman (Jacq.) Prain revealed that infection may also occur on natural breakages along the top root especially at the junction of the top secondary roots. Such natural wounds may be due to mechanical pressure from the emergence of secondary root and were found to be pronounced after 5-7 days inoculation. Apparently, BEs-4 cells colonized these vulnerable areas through chemotactic response as a result of leakage of organic metabolites coming from the exposed cells. BEs-4 cells were also found to be present in the pores and on surfaces of the young nodule tissues developing from the said infection sites after 14 days, suggesting a continuous colonization from the outside even after initial nodule formation.
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