Electrophoretic analysis of cationic proteins extracted from aflatoxin-resistant/susceptible varieties of corn.
1992
Neucere J.N.
The cationic proteins from two varieties of corn, Zea mays L., that are resistant (Yellow Creole) and susceptible (Huffman) to aflatoxin contamination were characterized by electrophoresis. Under native conditions, cathodic electrophoresis showed a cluster of proteins at Rf = 5.0-6.5 in Yellow Creole that was not evident in Huffman. SDS -PAGE showed approximately 12 bands that ranged between 14 and 43 kDa in both varieties. The profiles were different, both qualitatively and quantitatively. The major proteins in Yellow Creole clustered around 20 kDa on the gel and those in Huffman around 25 kDa. Qualitative variations of proteins by two-dimensional electrophoresis were evident, especially within the pH range 6.7-8.0 and around 27 and 92 kDa. The results are discussed in connection with the possible role of polypeptides as antifungal agents in species of corn.
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