Production of extracellular amylase by Bacillus thuringiensis subsp. kurstaki HD-1 and its characteristics.
1989
Kim S.Y. | Lee Y.C. | Lee H.H. | Yoo K.H.
The extracellular amylase production by Bacillus thuringiensis subsp. kurstaki HD-1 in amylase production media and its characteristics were investigated. The amylase production was highest in the medium composed of 0.2 % soluble starch, 1.0 % Bacto-peptone, 0.3 % beef extract, 0.3 % yeast extract, 0.5 % NaCl, 0.3 % K2HPO4, 0.1 % KH2PO4, 0.012 % CaCl2.2H2O, 0.005 % MnSO4. H2O, and 0.03 % MgSO4.7H2O). The amylase activity was inhibited by 50mM EDTA. The enzyme was optimally active from pH 6.5 to 7.0 at 55deg C. The specific activity of the enzyme in the ethanol precipitate was 2.01 units/mg, and the Km value approximately 0.8 mg/ml.
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