Microtubule distribution in unequal cell division of the grasshopper neuroblasts.
1990
Yamashiki N.
Microtubule distribution of grasshopper neuroblasts, which repeat unequal cell division, were demonstrated by indirect immunofluorescence microscopy. Immunoblot staining showed that both polyclonal antibody against bovine brain tubulin and monoclonal antibody against yeast tubulin used in this study cross-reacted with the grasshopper tubulin. The microtubules were organized to form the fibrous structure of the mitotic apparatus from late prophase till late telophase. From interface till middle prophase, a small number of microtubules were seen distributed within the nuclear core, and were resistant to colcemid treatment which depolymerized mitotic microtubules. The sizes of the mitotic asters at the two spindle poles were equal from late prophase till middle anaphase, and the configuration became different at late anaphase when the spindle shifting to eccentric position was completed. The results suggest that the spindle shifting is not brought about by the differential growth of the asters between the two poles.
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