Rozliseni odrud cicorky pestre (Coronilla varia) pomoci elektroforezy proteinu a enzymu.
1996
Sykorova S. | Sasek A. | Hofbauer J.
Electrophoretic analyses of seed proteins and seed enzymes were performed in three crown vetch (Coronilla varia) varieties: Kompolti, Eroza and Penngift to find the possibility of their mutual distinguishing. The qualitative difference was found in esterase isoenzymes between Kompolti and Eroza varieties after PAGE (polyacrylamide gel column electrophoresis) at pH 8.9 and the quantitative one between them after slab PAGE of negative proteins under the same conditions. No differences were found between Kompolti and Eroza varieties by SDS-PAGE of seed proteins; on the other hand, all procedures of protein and acid phosphatase electrophoresis documented the dissimilarity of American variety Penngift from Kompolti and Eroza varieties. According to these results Kompolti and Eroza varieties are in close relation to each other but distinguishable by means of negative seed protein and esterase isoenzyme electrophoresis. Penngift variety is considerably different from two former ones.
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