Optimization of electrochemiluminescence immunoassay for sensitive bacteria detection.
1997
Yu H.
Electrochemiluminescence Immunoassay (ECLIA) offers high sensitivity for detection of antigens in solution. However, conventional ECLIAs developed for bacteria detection are not optimal and many factors which may affect the ECLIA results still remain unclear. In this report, ECLIA kinetics, antibody biotinylation and non-specific binding were investigated. Results of these studies showed that the system optimization in ECLIA could substantially increase detection limit by at least 10-fold compared to conventional ECLIA.
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