DNA-fingerprinting with non-radioactive probe in a flounder, Paralichthys olivaceus.
1993
Hara M. | Dewa K. | Naito E. | Yamanouchi H.
DNA-fingerprinting with non-radioactive probe was applied to individual identifications in a flounder, Paralichthys olivaceus. (1) A human genome-deviced minisatellite, called myoprobe, could be used for DNA-fingerprinting of a flounder. (2) DNA for analysis of DNA-fingerprinting were extracted from as little as 0.1 ml blood of a flounder or less. (3) Individuals in a flounder were performed by means of DNA-fingerprinting with non-radioactive probe. (4) The DNA-fingerprinting of a flounder which was established on this experiment does not need the radioactive substance as marker attached to the probe. Therefore, it is considered that this method is applied in many laboratories without radio isotope equipment.
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