Monoclonal antibodies-based immunofluorescence test for detection of conidia of Botrytis cinerea on cut flowers.
1994
Salinas J. | Schots A.
Whole conidia, their extracellular material, and a putative cutin esterase isolated from conidia of Bortrytis cinerea were used as antigens to raise monoclonal antibodies (MAbs) for the detection of conidia of B cinerea. Using immunofluorescence, three selected MAbs recognized conidia of 43 isolates of B. cinerea from hosts representing six countries. The percentage of conidia that fluoresced ranged from 50 to 100%. Intensity of fluorescence was related more to the MAb than to the Botrytis isolate tested. MAbs showed no reaction with healthy Berbera flowers or to spores produced by other common airborne fungi and bacteria. Cross-reaction with conidia of four other species of Botrytis occurred, but their fluorescence patterns differed from those of conidia of B. cinerea.
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