Studies on simplified procedures for freezing and thawing of bovine embryos, 1; effects of PMSG and HCG injection on the induction of superovulation in rabbit.
1988
Kim J.K. | Kim C.K. | Lee K.H. | Kim D.C. | Kang M.S.
Effects of the injection levels of PMSG and HCG and injection time on the induction of superovulation of rabbits were determined. In experiment 1 adult female rabbits were intramuscularly injected with 200 IU, 50 IU per day for consecutive 2 days (100 IU) or 100 IU of PMSDG, respectively. HCG were injected to all rabbits 48 hrs after the PMSG. In experiment 2 rabbits were injected 100 IU of PMSG and 100 IU HCG (48 hrs after the PMSG) at the stage A(+), B(++) and C(+++); divided according to the degree of vaginal redness. Rabbits were allowed to mate and their reproductive organs were surgically removed 48 to 50 hours after mating. The size of ovaries, the number of follicles and hemorrhagic follicles, ovulation points and the recovery of embryos were determined. The number of follicles (hemorrhagic follicles) obtained in rabbits injected with 200 IU, 50 IU twice or 100 IU of PMSG was 21.5(42.2), 22.7(10.8) or 20.9(16.2), respectively. The size of ovaries was not different between rabbits injected with 100 IU of PMSG at vaginal stage A, B and C. However, the number of follicles (hemorrhagic follicles) was greater in rabbits injected at stage C than in those injected at stage A or B. The number of ovulation points (recovery rate of embryos) in rabbits injected with 200 IU, 50 IU twice or 100 IU of PMSG was 23.2(94%), 16.3(81%) or 22.1(86%), respectively. The number of ovulation points (recovery rate of embryos) in rabbits injected with 100 IU of PMSG at vaginal stage A, B or C was 32.4(91%), 18.0(84%) or 9.8(76%), respectively. The results indicate that the single injection of 100 IU PMSG at vaginal stage A was the best in terms of the follicle quality, the ovulation points and the recovery of embryos.
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