An effective low-cost micropropagation system in Tylophora indica.
1991
Bera T.K. | Roy S.C.
An effective low-cost micropropagation method was found out in Tylophora indica. The plant has been regenerated both directly and indirectly. In the direct method it was regenerated both from stem segments with pre-existing shoot buds as well as from leaf tissues. In the indirect method it was regenerated from callus, which was originated from leaf/stem explants. MS basal medium with different combination of auxins and cytokinins was used. Commercial grade sugar and single distilled water were used in all the methods. The result is based on the following aspects: (i) the total number of plants regenerated from a single explant; (ii) duration of regeneration (iii) frequency of regeneration; (iv) number of subculture needed to get the regenerated plant; (v) costs of chemicals used. The overall cost has been calculated in all methods of propagation and it was found that the direct way of micropropagation from leaf segments was the most useful low-cost method in this plant material.
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