Biological production of 2,3-butanediol from renewable biomass.
1997
Gong C.S. | Cao N. | Tsao G.T.
A simple and effective method of treatment of lignocellulosic material was used for the preparation of poplar wood chip and corn cob for the production of 2,3-butanediol by Klebsiella oxytoca ATCC 8724 in a simultaneous saccharification and fermentation (SSF) process. During the treatments, lignin and hemicellulose fractions of lignocellulosic materials were sequentially removed by aqueous ammonia (10-20%) steeping at 24 degrees C for 24 h followed by dilute hydrochloric acid (1%, w/v) hydrolysis at 100-108 degrees C for l h. The cellulose fractions (80 g/L) were then converted to butanediol by K. oxytoca in the presence of a fungal cellulase (8.5 g IFPU per g cellulose). The butanediol concentrations of 24 and 25.5 g/L, and ethanol concentrations of 6 and 7 g/L were produced by K. oxytoca from wood chip and corn cob, respectively. The average butanediol volumetric productivity was 0.26 g/L/h from wood chip and 0.35 g/L/h from corn cob.
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