Optimization of conditions for production of starter inoculum of Glomus mosseae Nic. and Gerd. (Gerd and Trappe).
1994
Brown M.B. | Luis E.M. | De Castro A.M. | Ilag L.L.
This study was conducted to develop a method for the production of VAM starter inoculum using Leonard jar and plastic tumbler. The set up was maintained at growth room conditions provided with light intensity of 10-15 klux, photo period at 14 hrs a day and temperature at 25-30 deg C. Among the three host plants and potting media evaluated, corn and perlite combination was found superior for root inoculum production. The composition of the nutrient solution was found to have a great influence on morphological anatomy of mycorrhizal root inoculum and the number of infective propagules. At high concentrations of N and P neither arbuscules nor vesicles formed. Both become prominent with increasing dilution of these 2 elements in nutrient solution. Increasing amount of F, Ca, and Mg from 20 ppm to 60 ppm had no remarkable effect on VAM colonization. Larger vesicles and more abundant extracellular VAM hyphae were evident on VAM roots raised in plastic tumblers at nutrient level optimum for root inoculum production for each technique.
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