In vitro micropropagation of Allium giganteum R., 2: Embryoid and plantlet regeneration through the anther culture of Allium giganteum R.
1994
Inagaki N. | Matsunaga H. | Kanechi M. | Maekawa S.
Anthers in small flower buds of 2 to 5 mm n length were used for embryoid and plantlets regeneration by anther culture of Allium giganteum, when the bract enveloping a umbel of 4 to 5 cm in diameter, which consisted of a number of small flower buds, was tearing. 1) Better embryoid regeneration (20 to 23%) from anther cultures was observed at the developmental stage of anther ranging from pollen mother cell to pollen tetrad on the medium containing 0.1 to 1 mg/1 BA (6-benzylaminopurine) and 10 mg/1 NAA (alpha-naphthalene acetic acid). Few embryoids were regenerated from anthers at uninuclear developmental stage. Embryoids obtained under the dark condition were developed into plantlets when transferred under the light condition. 2) The cold temperature pre-treatment at 5 degrees C for 5 to 10 days resulted in enhanced embryoid regeneration, and no embryoid regeneration in the case of one day pre-treatment. 3) Embryoids of smaller than 5 mm in length developed into normal plantlets when transferred onto the medium with 0.1 mg/1 IBA, while embryoids of larger than 5 mm abnormally developed into enlarged shoot and plantlets which were of glassy and succulent. 4) Most plantlets obtained through the anther culture were diploid. It has not been detected whether the plantlets originated in generative or vegetative cell tissues.
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