Genetic polymorphism of coconut (Cocos nucifera L.) varieties Catigan, Laguna and their F1 hybrid based on random amplified polymorphic DNA markers.
1996
Bebing N.N.
Genetic variations within and between coconut varieties Catigan (CAT), Laguna (LAG) and their CAT x LAG hybrid were determined by RAPD [random amplified polymorphic DNA] analysis. Coconut genomic DNA from leaf tissues were extracted using a small scale CTAB extraction method. RAPD experiments were carried out using 5 random primers and PCR [polymerase chain reaction] reagents from GIBCO-BRL. An optimized RAPD protocol was used for single and 2 primer RAPD analysis of coconut samples. DNA fragments were detected by silver staining. Four random primers and 7 combinations of two primers generated 59 RAPD markers which are then used to descriminate the coconut types based on their similarities and variations. Cluster analysis using the UPGMA [unweighted pair group method with arithmetic mean] method revealed a high degree of homogeneity within the CAT samples. Variations were high within LAG and CAT x LAG. Hybrid coconuts resembled more of the tall LAG variety than the CAT dwarfs. RAPD analysis was able to detect polymorphisms in these coconut types and confirmed the extent of variation within the LAG variety.
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