Studies on the development of new ornamental Allium through interspecific hybridization, 3: Hybridization of autumn-flowering species through pull-style pollination, cutflower culture and embryo rescue.
1994
Dubouzet J.G. | Arisumi K. | Etoh T. | Maeda M. | Sakata Y.
A pull-style pollination technique designed to avoid stylar incongruity was observed to increase the number of hybrid seeds produced per pollinated inflorescence by 31% relative to the standard pollination procedure. Compared to uncut inflorescences, cutflower culture implemented within 14 days from pollination, which was designed to avoid putatively unfavorable stimuli emanating from the bulb during embryogenesis, improved the fraction of viable embryos per pollinated ovule by 367%. Using the pull-style pollination, cutflower culture and embryo rescue techniques, we obtained 95 transplantable putative trispecific hybrid seedlings using the Yoshitake hybrids as maternal parents. The successful pollinator parents included a. tuberosum "Tenderpole", A cowanii, A. giganteum and the commercial cultivar "Murasame".
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