Survival of blue-green algae in various carrier media.
1986
Kotb S.I. | Angle J.S.
Three carrier media were studied to determine the most conducive to the survival of blue-green algae used to fix atmospheric nitrogen in paddy fields. Stored inoculum must remain viable for a minimum of 6 months. Three species of blue-green algae, Anabaena oryzae (Fritsch), Nostoc muscorum (Ag.) and Tolypothrix tenuis (Kutz.), were grown in modified Watanabe medium, harvested after 30 days of growth and added to the carrier media to determine the survival of the blue-green algae with time on the carriers. The survival of lyophilized algae was also examined. The inoculated carriers were stored in sealed containers at 25 degrees C and tested monthly for the number of viable cells, rate of nitrogen fixation and dry weight of regenerated algae. Significant differences in the tested parameters of the inoculants were observed; clay was found to be the most effective carrier for maintaining the viability of the algae and sand was consistently observed to be the poorest. The lyophilization treatment was also found to be an effective treatment for maintaining the viability of the algae.
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