Cloral micropropagation of Moringa oleifera L.
2010
Marfori, E.C., Philippines Univ. Los Baños, College, Laguna (Philippines). National Inst. of Molecular Biology and Biotechnology
A clonal in vitro micropropagation method of Moringa oleifera L. initiated from modal sections of young, aseptically-grown seedlings is described. Axillary shoot growth was induced by supplementing niura shige and Skoog's (MS) medium with a cytokinin. Of the three cytokinins tested, namely benzylaminopurine(BAP), Kinetin(Kin) and thidiazuran(TDZ), BAP at 2.5 micro M was found to be optimal in inducing bud break, producing an average of 4.6 axillary shoots per explant after 2 weeks. Through routine subculturing of nodal sections explanted from in vitro shoot cultures on a similar shoot induction medium, a high multiplication rate was established. Optimal rooting of individual shoot culture was obtained with application of naphthaleneacetic acid(NAA) at 0.25 micro M. Eighty percent of the rooted plants survived after being transplanted in the soil, provided that the potted plantlets were covered with clear polythene bags and kept in a shaded greenhouse for 2-4 wk before exposure to ambient conditions. In terms of growth and morphology, no abnormality was observed when the tissue culture-derived plants were compared with the mother plant.
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