Plantlet regeneration from cell suspension cultures of banana cv. saba via somatic embryogenesis
2009
Aspuria, E.T., Philippines Univ. Los Baños, College, Laguna (Philippines). Crop Science Cluster | de Juras, R.J., Philippines Univ. Los Baños, College, Laguna (Philippines). Crop Science Cluster
Higher number of meristematic buds(scalps) proliferated at the basal region of sterile shoot tip cultures in modified Murashige and Skoog's (1962) basal medium supplemented with 1 UM indoleacetic acid(IAA) and 100 uM benzylaminopurine(BA) in less than 3 weeks of incubation than those in media with lower BA concentrations. On the other hand, higher number of scalps was obtained in 1 uM thidiazuron(TDZ) than in BA (22.2 uM) supplemented culture medium. Yellowish and glossy meristematic globules were formed from enlarged scalps in modified liquid medium consisting of 1/2 Murashige and Skoog (MS) macro and iron-ethylenediaminetetraacetic acid (Fe-EDTA) with 5 uM 2,4-dichlorophenoxyacetic acid(2,4-D) and 1 uM Zeatin. Browning of scalp was effectively reduced by refreshing the culture medium every 2 days for the first 2 weeks of culture. Embryogenic cell suspension cultures released from meristematic globules were induced to undergo somatic embryogenesis in liquid MS medium supplemented with 9.1 uM Zeatin. Primary embryo development was already evident after 2 weeks of incubation. Primary embryo that were transferred onto semi-solid MS+9.1 uM Zeatin formed secondary embryos. Increased rate of shoot multiplication were observed on germinated secondary embryos which were passed through four subcultures onto MS+1 uM IAA+22.2 uM BA and further subcultured in MS+15% coconut water+22.2 uM BA (MC+22.2 uM BA).
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