Production of antagonistic actinomycetes against Fusarium oxysporum by solid state fermentation
2009
Lolium, B. | Chaiyanan, S.
The Streptomyces sp. isolate 22.2 was isolated from termite mound and proved to have antagonistic effect against Fusarium oxysporum that caused Fusarium wilt in solanaceous crops (tomato, potato, pepper, and eggplant). The antagonist isolate 22.2 had chitinolytic activity, therefore, chitin could be used as their carbon source in culture medium. The biomass production of antagonistic actinomycetes for using as a plant pathogen fungal control agent could be possible by the utilization of shellfish processing wastes. Shrimp shell powder prepared from dried shrimp processing waste was used as a major carbon source in culture medium. For optimization of solid-state fermentation of the antagonist, response surface methodology based on central composite design (CCD) was used. The design was employed by selecting ratios of shrimp shell powder-to-rice bran for being carbon sources in solid medium that having soy bean meal as nitrogen source at the ratio of carbon and nitrogen source 10:1, moisture content, and incubation time as model factors. The maximum yield of the antagonistic actinomycetes biomass derived from the optimized solid state fermentation using shrimp shell powder-to-rice bran ratio, 1; moisture level, 60%; and incubation time, 5 days was 7.5-8.0 log cfu/g dry matter.
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