Development of Bacillus amyloliquefaciens KPS46 formulation for control of soybean disease
2009
Preecha, C., Rajamangala Univ. of Technology, Srivijaya, Thung Song Nakhonsrithammarat, 80110 (Thailand). Dept. of Plant Science | Prathuangwong, S., Kasetsart Univ., Chatuchack, Bangkok 10900 (Thailand). Dept. of Plant Pathology
Bacillus amyloliquefaciens strain KPS46 had been reported to secrete antimicrobial metabolites and induce systemic resistant to the host plant. The additional based formulation advantages to withstand environmental stress and increase the survival rate and control efficacy stability of biological control bacteria was developed. The ratio of soybean meal:molasses and fish meal:molasses tested for enhancing multiplication for mass production was investigated. The optimal ratio was 10:5 g/l which costs calculated from all ingredient materials were 0.10 and 0.12 US$ per 10-liter. It cost lower than nutrient glucose broth of 13.58 US$ per 10-liter. The dry formulation should be maintained or increased its efficacy that was developed by using several local materials as carrier to reduce cost and enhance valuable of production. Viability investigated after 360-day shelflife of KPS46 maintained in wettable formula used rice husk ash dust, dry cow dung, decomposed cow dung, and talcum as carrier storage at room temperature were 8.8, 8.7, 8.5, and 8.4 log CFU respectively. Greenhouse and field experiment of KPS46 developed as wettable formula could reduce Sclerotium damping-off, bacterial pustule caused by Xanthomonas axonopodis pv. glycines and anthracnose caused by Colletotrichum truncatum. It promoted plant growth of green soybean under field experiment to increase quality and quantity of marketable yield with 24.1 and 29.5% compared with non-treated control for the first crop and the second crop, respectively. The return on investment was 43.30% higher than conventional of 33.59%.
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