Assessment of genetic variability in sorghum genotypes for drought tolerance based on RAPD analysis
2011
Akram, Z. (PMAS Arid Agriculture Univ., Rawalpindi (Pakistan)) | Khan, M.M. (PMAS Arid Agriculture Univ., Rawalpindi (Pakistan)) | Shabbir, G. (PMAS Arid Agriculture Univ., Rawalpindi (Pakistan)) | Nasir, F. (PMAS Arid Agriculture Univ., Rawalpindi (Pakistan))
In a study conducted in the Department of Plant Breeding and Genetics, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan during the year 2008-09, 20 sorghum (Sorghum bicolor) genotypes were evaluated for their genetic variability in drought tolerance. These genotypes were analyzed using 10 RAPD primers. Total number of loci traced by these primers was 66, of which all were polymorphic showing 100 percent polymorphism. Size of score able fragments ranged from 250-3000 bp. The genetic distance ranged from 51.5 to 95.5 percent for drought tolerance. The results obtained from RAPD showed 99.5 percent overall polymorphism among 20 genotypes for concerned trait. Genotypes were grouped in cluster by using cluster analysis and most diverse genotypes were identified. These results confirmed the availability of maximum amount of genetic variability for drought tolerance in the selected genotypes.
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