Computational identification of miRNAs and their targets from Crocus sativus L.
2012
Guleria, P., Council of Scientific and Industrial Research, Palampur, HP (India). CSIR-Institute of Himalayan Bioresource Technology, Biotechnology Division | Goswami, D., Council of Scientific and Industrial Research, Palampur, HP (India). CSIR-Institute of Himalayan Bioresource Technology, Biotechnology Division | Yadav, S.K., Council of Scientific and Industrial Research, Palampur, HP (India). CSIR-Institute of Himalayan Bioresource Technology, Biotechnology Division
miRNAs are smaller known entities that perform several crucial regulatory roles in plants. Identification and characterization of miRNAs has been a challenging task which has become simplified with the development of computational approaches. In the present study, three novel miRNAs were predicted from Crocus sativus by computational approaches. A total 6767 ESTs were mined from the EST database available at NCBI. The software MicroPC was utilized which resulted with the prediction of three miRNAs, csa-miR1, csa-miR2 and csa-miR3. Targets were predicted for the respective miRNAs using the miRU2 program. The identified targets have a role in plant growth, senescence, disease resistance and various stress responses. In addition, some targets are involved in mRNA export, protein synthesis and posttranslational modifications.
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