The effect of pre-treatment with L-NAME on glutathione and glutathione peroxidase in parquat-induced neurotoxicity in rat striatum | Efekat predtretmana sa L-NAME na glutation i glutation peroksidazu u strijatumu pacova na neurotoksičnost izazvanu parakvatom
2012
Đukić, M., Farmaceutski fakultet, Beograd (Serbia) | Ninković, M., Vojnomedicinska akademija, Beograd (Serbia). Institut za medicinska istraživanja | Stevanović, I., Vojnomedicinska akademija, Beograd (Serbia). Institut za medicinska istraživanja | Ilić, K., Farmaceutski fakultet, Beograd (Serbia) | Đurđević, D., Vojnomedicinska akademija, Beograd (Serbia). Institut za medicinska istraživanja
Contact herbicide paraquat (DQ) is bipyridylium compound, which undergoes redox metabolism; hence enlarged in humans radical production mediated its toxicity. Target organs of systemic effect of PQ poisoning are lung and kidney. The mechanism of PQ-induced neurotoxicity is not elucidated till now. The objective of our study was to examine the role of nitric oxide (NOx) in PQ-induced neurotoxicity, primarily focusing on glutathione cycles [total glutathione content (GSH) and glutathione peroxidase (GPx) activity]. In order to investigate the role of NOx in oxidative stress (OS) and/or nitrosative stress (NS) response to PQ neurotoxicity, we used NG-nitro-L-arginine methyl ester (L-NAME), a non-selective inhibitor of nitric oxide synthase (NOS) in the pretreatment of PQ administration. Study was conducted on Wistar rats randomly divided in groups (n=8 for controls and n=24 for experimental groups) depending on the applied treatments. The tested compounds were intrastriatally (i.s.) administered. Measuring of GSH content and GPx activity was performed at 30 min, 24 hours and 7 days after treatments. Parkinsonism’s like symptoms were observed only in the group of rats treated with PQ. The L-NAME protected animals from PQ-induced neurotoxicity, which could be concluded from the absence of Parkinsonism’s like symptoms and reduced OS/NS response in the striatum of rats pretreated with L-NAME.
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